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KMID : 0380020080230020135
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 2 p.135 ~ p.141
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Simulation of SMB [Simulated Moving Bed] Chromatography for Separation of L-ribose and L-arabinose by ASPEN chromatography
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Lee Seon-Hee
Kim In-Ho
Lee Eun
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Abstract
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SMB (simulated moving bed) chromatography is a very useful utility for the separation of binary system. We simulated the separation of L-arabinose and L-ribose from the mixture by using lab-scale 4(1-1-1-1)-zone SMB chromatography. Preliminary experiments of PIM (pulse input method) were performed to measure adsorption isotherms of L-ribose and L-arabinose in HPLC column, and experimental and simulated results from ASPEN chromatography were compared. To find the most suitable separation condition in SMB, we carried out a simulation in plane base on the triangle theory and calculated operating parameters (flow rate of four zone, switching time and feed concentration and so on) using ASPEN chromatography under the conditions of linear isotherms obtained from PIM.
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KEYWORD
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SMB, simulated moving bed, chromatography, ASPEN chromatography, L-arabinose and L-ribose
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